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 >  Protein>IL-13 R alpha 1 >IL1-H82F6

Biotinylated Human IL-13 R alpha 1 Protein, Fc,Avitag™ (SPR verified)

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分子别名(Synonym)

IL13RA1,CD213A1,IL-13Ra,NR4,RP13-128O4.2,IL13Rα1

表达区间及表达系统(Source)

Biotinylated Human IL-13 R alpha 1, Fc,Avitag (IL1-H82F6) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Thr 343 (Accession # NP_001551.1).

Predicted N-terminus: Gly 22

Request for sequence

蛋白结构(Molecular Characterization)

Online(Gly 22 - Thr 343) NP_001551.1

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 65.0 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human IL-13 R alpha 1, Fc,Avitag (Cat. No. IL1-H82F6) SDS-PAGE gel

Biotinylated Human IL-13 R alpha 1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

活性(Bioactivity)-SPR

Biotinylated Human SPR

Biotinylated Human IL-13 R alpha 1, Fc,Avitag (Cat. No. IL1-H82F6) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-13, His Tag (Cat. No. IL3-H52H4) with an affinity constant of 45.6 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Protocol

 

背景(Background)

Interleukin 13 receptor, alpha 1 is also known as IL13RA1, NR4 and CD213A1 (cluster of differentiation 213A1), The IL13 Rα1 cDNA encodes a 427 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 324 aa residue extracellular domain, a 23 aa residue transmembrane region and a 59 aa residue cytoplasmic tail. Human and mouseIL13Rα1 share 76% aa sequence identity. IL13RA1 is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.

 

 

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IL-13 R alpha 1靶点信息
英文全称:Interleukin-13 receptor subunit alpha-1
中文全称:白介素-13受体α1亚基
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床二期
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