膜杰作
Star Staining
分子别名（Synonym）
C7orf15,C7orf15MGC138295,CD112R,MGC104322,MGC138297,MGC2463,PVRIG,CD112 receptor
表达区间及表达系统（Source）
Biotinylated Human PVRIG, Fc,Avitag (PVG-H82F4) is expressed from human 293 cells (HEK293). It contains AA Thr 41 - Asp 171 (Accession # Q6DKI7-1).
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 41.9 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human PVRIG, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F4) is more than 90% and the molecular weight of this protein is around 82-110 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F4) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Nectin-2, Mouse IgG2a Fc Tag (Cat. No. CD2-H5257) with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F4) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Nectin-2, His Tag (Cat. No. PV2-H52E2) with a linear range of 0.02-0.313 μg/mL (Routinely tested).
Protocol
Immobilized Human Nectin-2, His Tag (Cat. No. PV2-H52E2) at 10 μg/mL (100 μL/well) can bind Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F4) with a linear range of 0.005-0.313 μg/mL (Routinely tested).
Protocol
Immobilized Human Nectin-2, Fc Tag (Cat. No. PV2-H5253) at 10 μg/mL (100 μL/well) can bind Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F4) with a linear range of 0.005-0.313 μg/mL (Routinely tested).
Protocol
Immobilized Human Nectin-2, Mouse IgG2a Fc Tag (Cat. No. CD2-H5257) at 10 μg/mL (100 μL/well) can bind Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F4) with a linear range of 0.01-0.313 μg/mL (Routinely tested).
Protocol
背景（Background）
Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family. The CD112R gene encodes a putative single transmembrane protein, which is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. Notably, the intracellular domain of phatases. The extracellular domain sequence of human and mouse CD112R have 65.3% similarity. CD112R may act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals.
