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 >  Protein>FcRn (FCGRT & B2M) >FCM-C82W5

Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag™&Strep II Tag (HPLC&SPR&BLI verified)

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分子别名(Synonym)

FcRn,FCGRT & B2M

表达区间及表达系统(Source)

MABSol® Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag (FCM-C82W5) is expressed from human HEK293 cells. It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q8SPV9-1 (FCGRT) & Q8SPW0-1 (B2M)). In the region Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M), the AA sequence of Cynomolgus and Rhesus macaque FcRn (FCGRT & B2M) are homologus.

Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)

Request for sequence

蛋白结构(Molecular Characterization)

FcRn (FCGRT & B2M) Structure

Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.1 kDa (FCGRT) and 13.0 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with a Strep II tag at the C-terminus. The reducing (R) protein migrates as 40 kDa (FCGRT) and 14 kDa (B2M) respectively due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

FcRn (FCGRT & B2M) SDS-PAGE

Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-HPLC

FcRn (FCGRT & B2M) SEC-HPLC

The purity of Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag (Cat. No. FCM-C82W5) was greater than 85% as determined by SEC-HPLC.

 

活性(Bioactivity)-SPR

FcRn (FCGRT & B2M) SPR

Captured Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag (Cat. No. FCM-C82W5) on Biotin CAP - Series S sensor Chip can bind Herceptin® with an affinity constant of 0.241 μM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

 

活性(Bioactivity)-BLI

FcRn (FCGRT & B2M) BLI

Loaded Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag (Cat. No. FCM-C82W5) on SA Biosensor, can bind Herceptin with an affinity constant of 0.075 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

背景(Background)

FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

 

文献引用(Citations)

 

前沿进展

 
 
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FcRn (FCGRT & B2M)靶点信息
英文全称:IgG receptor FcRn large subunit p51
中文全称:IgG受体FcRn大亚基p51
种类:Homo sapiens
上市药物数量:3详情
临床药物数量:3详情
最高研发阶段:批准上市
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