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Fluorescent Human Claudin-18.2 Full Length Protein-VLP (HEK293)

分子别名(Synonym)

Claudin-18.2,CLDN18,Claudin-18

表达区间及表达系统(Source)

Fluorescent Human Claudin-18.2 Full Length Protein-VLP (CL2-HF218) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Val 261 (Accession # P56856-2).

Predicted N-terminus: Met 1

蛋白结构(Molecular Characterization)

This protein carries a GFP tag at the C-terminus.

Virus-like particles(VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrance Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.

The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.

Conjugate

GFP

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 530 nm

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

制剂(Formulation)

Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 12 months under sterile conditions.
*The isotype control of empty/mock VLP (Cat. No. VLP-NF2P4) is sold separately and not included in protein, you can follow this link for product information.
 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Fluorescent Human Claudin-18.2 Full Length Protein-VLP (HEK293) (Cat. No. CL2-HF218) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Fluorescent Human Claudin-18.2 Full Length Protein-VLP (Cat. No. CL2-HF218) captured on CM5 Chip via Anti-Claudin-18.2 antibody can bind Anti-Claudin-18.2 antibody with an affinity constant of 0.433 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-FACS

Biotinylated Human FACS

2e5 of Anti-Claudin-18.2 CAR-293 cells were stained with 100 μL of 3 μg/mL of Fluorescent Human Claudin-18.2 Full Length Protein-VLP (Cat. No.CL2-HF218) and negative control protein respectively, FITC signals was used to evaluate the binding activity (QC tested).

Protocol

 
 

均一性(Identity)-DLS

Identity DLS

The mean peak Radius of VLP is 65-85 nm with more than 95% intensity as determined by dynamic light scattering (DLS).

背景(Background)

Claudins (CLDNs) are a family of proteins that form tight junctions and maintain the polarity of epithelial and endothelial cells. CLDN18 is specifically expressed in the stomach and lung. Of the two CLDN18 isoform transcripts produced by alternative splicing, CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific. CLDN18.2 is a highly selective gastric lineage antigen expressed exclusively on short-lived differentiated gastric epithelial cells where it has only limited accessibility to antibody drugs.14,15 CLDN18.2 is maintained during the course of malignant transformation and thus frequently displayed on the surface of human gastric cancer cells.

 

前沿进展

 
 
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400-682-2521(全国)
010-53681107(北京)
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技术支持邮箱:
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Claudin-18.2靶点信息
英文全称:Claudin-18 splice variant 2
中文全称:密蛋白-18剪接变体2
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:24详情
最高研发阶段:临床三期
前沿进展

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