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NFAT (Luc) HEK293 Reporter Cell

For research use only.

描述(Description)

The NFAT (Luc) HEK293 Reporter Cell was engineered with the NFAT response element driving luciferase expressing systems. The receptors expressing endogenously or transfected on this reporter cell were activated by corresponding ligands binding, transducing intracellular signals resulting in NFAT-RE mediated luminescence.

应用说明(Application)

1. The discovery of activators or inhibitors by the NFAT signaling bioactivity

2. Transfection host for some receptors concerning the NFAT signaling pathway

生长特性(Growth Properties)

Adherent

筛选标记(Selection Marker)

Hygromycin (50 μg/mL)

培养基(Culture Medium)

DMEM medium + 10% FBS

冻存液(Freeze Medium)

Serum-free cell cryopreservation medium

装量(Quantity)

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

存储(Storage)

Frozen in liquid nitrogen.

支原体检测(Mycoplasma Testing)

Negative

无菌检测(Sterility Testing)

Negative

使用说明(Instructions for Use)

See data sheet for detailed culturing and assay protocol.

 

Signaling Bioassay

NFAT SIGNALING

Response to PMA plus Ionomycin (RLU).
The NFAT (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of PMA plus Ionomycin (2 μM). The EC50 was approximately 3.4 ng/mL.

Protocol

NFAT SIGNALING

Response to PMA plus Ionomycin (Fold).
The NFAT (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of PMA plus Ionomycin (2 μM). The max induction fold was approximately 430.

Protocol

 

Passage Stability

NFAT PASSAGE

Passage stability analysis by Signaling Bioassay.
The continuously growing NFAT (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of PMA plus Ionomycin (2 μM). PMA plus Ionomycin stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 25-35. And, the bioactivity of NFAT signaling still can be detected by PMA plus Ionomycin stimulation on passage 35 with high activating fold.

Protocol

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背景(Background)

Nuclear factor of activated T cells (NFAT), which is the pharmacological target of immunosuppressants cyclosporine and tacrolimus, has been shown to play an important role in T cells (immune system) and many biological events. Accumulating studies have indicated that NFATs are involved in many aspects of cancer, including carcinogenesis, cancer cell proliferation, invasion, metastasis, angiogenesis, drug resistance and tumor microenvironment. As the key section of the adaptive immune response, the T cell receptor (TCR)-calcium-calcineurin signaling pathway could lead to T cell activation. The 'nuclear factor of activated T cells' proteins are transcription factors that promote expression of a panel of genes required for activation.

 

License Disclosure

This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.

 

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