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 >  Protein>Thrombopoietin R >THR-H52H7

Human Thrombopoietin R / TPO R Protein, His Tag (MALS verified)

分子别名(Synonym)

MPL,C-MPL,CD110,MPLV,THCYT2,TPOR

表达区间及表达系统(Source)

Human Thrombopoietin R, His Tag (THR-H52H7) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Trp 491 (Accession # P40238-1 ).

Predicted N-terminus: Gln 26

Request for sequence

蛋白结构(Molecular Characterization)

Thrombopoietin R Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 54.4 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Thrombopoietin R SDS-PAGE

Human Thrombopoietin R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Thrombopoietin R SEC-MALS

The purity of Human Thrombopoietin R, His Tag (Cat. No. THR-H52H7) is more than 90% and the molecular weight of this protein is around 60-74 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-SPR

Thrombopoietin R SPR

Human Thrombopoietin R, His Tag (Cat. No. THR-H52H7) captured on CM5 Chip via anti-His antibody can bind Human Thrombopoietin, Tag Free (Cat. No. THN-H5214) with an affinity constant of 14.9 nM as determined in SPR assay (Biacore T200) (Routinely tested).

Protocol

 

背景(Background)

Thrombopoietin R, also known as TPO-R, is expressed predominantly on the surface of MKs, platelets, hemangioblasts, and hematopoietic stem cells (HSCs). Binding of TPO to the megakaryocyte TPO-R leads to different effects: prevention of megakaryocyte apoptosis; increased megakaryocyte number, size, and ploidy; increasing rate of megakaryocyte maturation; and internalization of the TPO/TPO-R complex. Thrombopoietin R involved in multiple signal transduction pathways, such as JAK, STAT, and MAP kinase.

 

前沿进展

 
 
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Thrombopoietin R靶点信息
英文全称:Thrombopoietin receptor
中文全称:血小板生成素受体
种类:Homo sapiens
上市药物数量:7详情
临床药物数量:13详情
最高研发阶段:批准上市
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