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Biotinylated Cynomolgus Transferrin R / CD71 Protein, His Tag, ultra sensitivity (primary amine labeling)

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分子别名(Synonym)

CD71,TFRC,TFR,,TfR1,Trfr,T9,p90

表达区间及表达系统(Source)

Biotinylated Cynomolgus Transferrin R, His Tag, primary amine labeling (TFR-C8249) is expressed from human 293 cells (HEK293). It contains AA Cys 89 - Phe 760 (Accession # G8F602-1 ). It is the biotinylated form of Cynomolgus Transferrin R, His Tag(Cat. No. TFR-C524a).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

Transferrin R Structure

This protein carries a polyhistidine tag at the N-terminus

The protein has a calculated MW of 77.1 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Transferrin R SDS-PAGE

Biotinylated Cynomolgus Transferrin R, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Transferrin R ELISA

Immobilized Biotinylated Cynomolgus Transferrin R, His Tag, primary amine labeling (Cat. No. TFR-C8249) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse Transferrin, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. TRN-M525b) with a linear range of 0.3-10 ng/mL (QC tested).

Protocol

Transferrin R ELISA

Immobilized Mouse Transferrin, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. TRN-M525b) at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus Transferrin R, His Tag, primary amine labeling (Cat. No. TFR-C8249) with a linear range of 0.3-10 ng/mL (Routinely tested).

Protocol

 

背景(Background)

CD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71 / TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE.

 

前沿进展

 
 
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Transferrin R靶点信息
英文全称:Transferrin receptor protein 1
中文全称:转铁蛋白受体1
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:9详情
最高研发阶段:批准上市
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