膜杰作
Star Staining
抗体来源（Source）
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) (SPD-M334) is a chimeric monoclonal antibody recombinantly expressed from HEK293 cells, which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody was obtained from a mouse immunized with recombinant SARS-CoV-2 Spike RBD protein. The antibody is specific against the Beta (B.1.351) and Gamma (P.1) variant of SARS-CoV-2, and has no binding with the spike RBD of the wild type virus, the Alpha (B.1.1.7) variant, the Delta (B.1.617.2) variant and the Omicron (B.1.1.529, BA.2) variant.
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
特异性（Specificity）
This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. No cross-reactivity is detected with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
电泳（SDS-PAGE）
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) (Cat. No. SPD-M334) is more than 90% and the molecular weight of this protein is around 130-155 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized SARS-CoV-2 spike protein (Beta, Cat. No. SPN-C52Hk and Gamma, Cat. No. SPN-C52Hg) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) (Cat. No. SPD-M334) with a linear range of 0.1-8 ng/mL. The antibody does not bind spike protein of WT (Cat. No. SPN-C52H7), Alpha (Cat. No. SPN-C52H6) and Delta (Cat. No. SPN-C52He) (QC tested).
Protocol
Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) (Cat. No. SPD-M334) can bind SARS-CoV-2 spike protein (Beta, Cat. No. SPN-C52Hk and Gamma, Cat. No. SPN-C52Hg) with a linear range of 0.5-8 ng/mL. The antibody does not bind spike protein of WT (Cat. No. SPN-C52H7), Alpha (Cat. No. SPN-C52H6) and Delta (Cat. No. SPN-C52He) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) (Cat. No. SPD-M334) loaded on AMC Biosensor can bind SARS-CoV-2 S protein, His Tag (Beta, Cat. No. SPN-C52Hk) with an affinity constant of 11.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) (Cat. No. SPD-M334) loaded on AMC Biosensor can bind SARS-CoV-2 S protein, His Tag (Gamma, Cat. No. SPN-C52Hg) with an affinity constant of 13.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) (Cat. No. SPD-M334) loaded on AMC Biosensor does not bind SARS-CoV-2 S protein, His Tag (WT, Cat. No. SPN-C52H7) as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) (Cat. No. SPD-M334) loaded on AMC Biosensor does not bind SARS-CoV-2 S protein, His Tag (Alpha, Cat. No. SPN-C52H6) as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
Its been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
