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 >  Protein>GPRC5D >GPD-H82D6

Biotinylated Human GPRC5D Protein, His,Avitag™&Flag Tag (Detergent)

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分子别名(Synonym)

GPRC5D

表达区间及表达系统(Source)

Biotinylated Human GPRC5D, His,Avitag&Flag Tag (GPD-H82D6) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Phe 192 & Glu 197 - Leu 262 (Accession # Q9NZD1-2 ).

Predicted N-terminus: Asp

Request for sequence

蛋白结构(Molecular Characterization)

GPRC5D Structure

This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus followed by an Avi tag (Avitag™).

The protein has a calculated MW of 53.5 kDa. The protein migrates as 35-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>80% as determined by SDS-PAGE.

制剂(Formulation)

This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.

Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 3 months under sterile conditions.
**The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow this link for product information.
 

电泳(SDS-PAGE)

GPRC5D SDS-PAGE

Biotinylated Human GPRC5D, His,Avitag&Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 80% (With Star Ribbon Pre-stained Protein Marker).

 

活性(Bioactivity)-ELISA

GPRC5D ELISA

Immobilized Biotinylated Human GPRC5D, His,Avitag&Flag Tag (Cat. No. GPD-H82D6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-GPRC5D Antibody, Human IgG4 with a linear range of 0.001-0.25 μg/mL (QC tested).

Protocol

 

活性(Bioactivity)-SPR

GPRC5D SPR

Anti-GPRC5D Antibody captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Biotinylated Human GPRC5D, His,Avitag&Flag Tag (Cat. No. GPD-H82D6) with an affinity constant of 0.112 μM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

GPRC5D BLI

Loaded Biotinylated Human GPRC5D, His,Avitag&Flag Tag (Cat. No. GPD-H82D6) on SA Biosensor, can bind Anti-GPRC5D Antibody, Human IgG4 with an affinity constant of 1.14 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

G-protein coupled receptor family C group 5 member D (GPRC5D) is a retinoic acid-inducible 40 kDa protein with seven transmembrane segments and a short N terminal extracellular region. Widely expressed in the peripheral system. Expression pattern is high in pancreas, medium in kidney, small intestine, spleen and testis, low in lung, colon, leukocyte, prostate and thymus and not detectable in brain, heart, liver, placenta, skeletal muscle and ovary.

 

前沿进展

 
 
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GPRC5D靶点信息
英文全称:G-protein coupled receptor family C group 5member D
中文全称:G蛋白偶联受体家族C组5成员D
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:17详情
最高研发阶段:批准上市
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