膜杰作
Star Staining
表达区间及表达系统（Source）
Human Neutrophil defensin 1, Twin-Strep Tag (ND1-H5282) is expressed from human 293 cells (HEK293). It contains AA Asp 39 - Cys 94 (Accession # P59665-1).
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蛋白结构（Molecular Characterization）
This protein carries a twin strep tag at the C-terminus
The protein has a calculated MW of 9.8 kDa. The protein migrates as 11-14 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human Neutrophil defensin 1, Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
背景（Background）
Human defensins are a family of small antimicrobial proteins found predominantly in leukocytes and epithelial cells that play important roles in the innate and adaptive immune defense against microbial infection. The α-defensins, human neutrophil peptides (HNPs) 1–3, are constitutively expressed in neutrophils and are the most abundant neutrophil granule proteins.
