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 >  Protein>IL-11 R alpha >ILR-H82F5

Biotinylated Human IL-11 R alpha Protein, Fc,Avitag™ (MALS verified)

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分子别名(Synonym)

IL-11 R alpha,IL-11 RA,IL11RA,Interleukin-11 receptor subunit alpha,IL-11 receptor subunit alpha,IL-11R subunit alpha,IL-11R-alpha

表达区间及表达系统(Source)

Biotinylated Human IL-11 R alpha, Fc,Avitag (ILR-H82F5) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Ala 370 (Accession # Q14626-1 ).

Predicted N-terminus: Ser 24

Request for sequence

蛋白结构(Molecular Characterization)

IL-11 R alpha Structure

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 66.0 kDa. The protein migrates as 70-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-11 R alpha SDS-PAGE

Biotinylated Human IL-11 R alpha, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

IL-11 R alpha SEC-MALS

The purity of Biotinylated Human IL-11 R alpha, Fc,Avitag (Cat. No. ILR-H82F5) is more than 90% and the molecular weight of this protein is around 140-175 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

IL-11 R alpha ELISA

Immobilized Human IL-11, His Tag (Cat. No. IL1-H5243) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-11 R alpha, Fc,Avitag (Cat. No. ILR-H82F5) with a linear range of 2-39 ng/mL (QC tested).

Protocol

 

背景(Background)

Receptor for interleukin-11 (IL11). The receptor systems for IL6, LIF, OSM, CNTF, IL11 and CT1 can utilize IL6ST for initiating signal transmission. The IL11/IL11RA/IL6ST complex may be involved in the control of proliferation and/or differentiation of skeletogenic progenitor or other mesenchymal cells (Probable). Essential for the normal development of craniofacial bones and teeth. Restricts suture fusion and tooth number.Soluble form of IL11 receptor (sIL11RA) that acts as an agonist of IL11 activity. The IL11:sIL11RA complex binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL11RA in a process called IL11 trans-signaling.

 

前沿进展

 
 
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IL-11 R alpha靶点信息
英文全称:Interleukin 11 receptor alpha
中文全称:白细胞介素-11受体α
种类:Homo sapiens
上市药物数量:9详情
临床药物数量:3详情
最高研发阶段:批准上市
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