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Human CD72 Protein, Mouse IgG2a Fc Tag (MALS verified)

分子别名(Synonym)

CD72,Lyb-2,Lyb2

表达区间及表达系统(Source)

Human CD72, Mouse IgG2a Fc Tag (CD2-H5255) is expressed from human 293 cells (HEK293). It contains AA Arg 117 - Asp 359 (Accession # P21855-1).

Predicted N-terminus: Arg 117

Request for sequence

蛋白结构(Molecular Characterization)

Online(Arg 117 - Asp 359) P21855-1

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 55.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD72, Mouse IgG2a Fc Tag (Cat. No. CD2-H5255) SDS-PAGE gel

Human CD72, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Human CD72, Mouse IgG2a Fc Tag (Cat. No. CD2-H5255) MALS images

The purity of Human CD72, Mouse IgG2a Fc Tag (Cat. No. CD2-H5255) is more than 85% and the molecular weight of this protein is around 108-132kDa verified by SEC-MALS.

Report

背景(Background)

B-cell differentiation antigen CD72 is also known as Lyb-2. CD72 contains one C-type lectin domain. CD72 is a protein active in the immune system of animals. CD72 consists of two identical halves, each of about 39-43 kD, and is a C-type lectin. Its primarily locus of expression is B-cells, where it appears to mediate aspects of B-cell - T-cell interaction. CD72 plays a role in B-cell proliferation and differentiation. CD72 is a ligand for CD5.

 

 

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