膜杰作
Star Staining
Aneuro
ComboX
ActiveMax
GENPower
ClinMax
Star Ribbon
分子别名（Synonym）
LRP-6,LRP6
表达区间及表达系统（Source）
Biotinylated Human LRP-6, His,Avitag (LR6-H82E7) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Pro 630 (Accession # O75581-1 ).
Predicted N-terminus: Ala 20
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 72.5 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Biotinylation）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Biotin:Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human LRP-6, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Human R-Spondin 1, His Tag at 1 μg/mL (100 μL/well) can bind Biotinylated Human LRP-6, His,Avitag (Cat. No. LR6-H82E7) with a linear range of 20-156 ng/mL (QC tested).
Protocol
背景（Background）
LRP6 is a transmembrane low-density lipoprotein receptor that shares a similar structure with LRP5. Low-density lipoprotein receptor-related protein 6 is a key component of the LRP5/LRP6/Frizzled co-receptor group that is involved in canonical Wnt pathway. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin.
