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 >  Protein>Post-Fusion glycoprotein F0 >RSF-V52H6

HRSV (A) Post-fusion glycoprotein F0, His Tag (MALS verified)

表达区间及表达系统(Source)

HRSV (A) Fusion glycoprotein F0, His Tag (RSF-V52H6) is expressed from human 293 cells (HEK293).

Predicted N-terminus: Gln 26

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 55.2 kDa. The protein migrates as 47-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

After reconstitution, this product is stable after storage at -70°C for 3 months.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. For long term storage, the product is stable for up to 3 years at -70°C from date of receipt;
  2. For short term storage, the product is stable for up to 12 months at 2-8°C from date of receipt.
 

电泳(SDS-PAGE)

Post-Fusion glycoprotein F0 SDS-PAGE

HRSV (A) Fusion glycoprotein F0, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Post-Fusion glycoprotein F0 SEC-MALS

The purity of HRSV (A) Fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6) is more than 90% and the molecular weight of this protein is around 165-185 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Post-Fusion glycoprotein F0 ELISA

Immobilized HRSV (A) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6) and HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) at 1 μg/mL (100 μL/well) can bind Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) with a linear range of 0.1-4 ng/mL (QC tested).

Protocol

Post-Fusion glycoprotein F0 ELISA

Immobilized HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) at 1 μg/mL (100 μL/well) can bind Recombinant Human Anti-RSV Antibody (AM14) with a linear range of 0.004-0.25 μg/mL. HRSV (A) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6) is verified not recoginized by Recombinant Human Anti-RSV Antibody (AM14) (QC tested).

Protocol

Post-Fusion glycoprotein F0 ELISA

Immobilized HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) at 1 μg/mL (100 μL/well) can bind Recombinant Mouse Anti-RSV F Antibody (5C4) with a linear range of 0.004-0.25 μg/mL. HRSV (A) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6) is verified not recoginized by Recombinant Mouse Anti-RSV F Antibody (5C4) (Routinely tested).

Protocol

Post-Fusion glycoprotein F0 ELISA

Immobilized HRSV (A) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6) and HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) at 1 μg/mL (100 μL/well) can bind Anti-RSV Fusion Protein Antibody (131-2A) with a linear range of 0.1-1 ng/mL (Routinely tested).

Protocol

Post-Fusion glycoprotein F0 ELISA

Immobilized HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) at 1 μg/mL (100 μL/well) can bind Recombinant Human Anti-RSV Antibody (D25) with a linear range of 0.1-2 ng/mL. HRSV (A) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6) is verified not recoginized by Recombinant Human Anti-RSV Antibody (D25) in low concentration (Routinely tested).

Protocol

Post-Fusion glycoprotein F0 ELISA

Immobilized HRSV (A) Post-fusion glycoprotein F0, His Tag (MALS verified) (Cat. No. RSF-V52H6) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-RSV-F0 broadly Antibody, Mouse IgG1 (3B4) (Cat. No. RS0-Y161) with a linear range of 0.144-9.197 ng/mL (Routinely tested).

Protocol

Post-Fusion glycoprotein F0 ELISA

Immobilized HRSV (A) Post-fusion glycoprotein F0, His Tag (MALS verified) (Cat. No. RSF-V52H6) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-RSV-F0 broadly Antibody, Mouse IgG1 (4G2) (Cat. No. RS0-Y162) with a linear range of 0.049-1.563 ng/mL (Routinely tested).

Protocol

 

背景(Background)

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. The RSV fusion glycoprotein (RSV F) is the principal target of RSV neutralizing antibodies in human sera. The RSV F is a type I viral fusion protein synthesized as inactive, single-chain polypeptides that assemble into trimers. RSV F fuses the viral and host cell membranes by irreversible protein refolding from the labile prefusion conformation to the stable post-fusion conformation. Both states exhibit epitopes targeted by neutralizing antibodies, and post-fusion RSV F is being developed as a vaccine candidate.

 

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