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Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS106) (Beta & Gamma Specific)

表达区间及表达系统(Source)

The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Spike RBD. The antibody is specific against the Beta (B.1.351) and Gamma (P.1) variant of SARS-CoV-2, and has no binding with the spike RBD of the wild type virus, the Alpha (B.1.1.7) variant and the Delta (B.1.617.2) variant.

亚型(Isotype)

Mouse IgG1/kappa

特异性(Specificity)

This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. No cross-reactivity is detected with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.

纯度(Purity)

>95% as determined by SDS-PAGE.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

运输(Shipping)

This product is supplied and shipped with dry ice, please inquire the shipping cost.

制剂(Formulation)

Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 3 months under sterile conditions.
 

电泳(SDS-PAGE)

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS106) (Beta & Gamma Specific) (Cat. No. SPD-M416) SDS-PAGE gel

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS106) (Beta & Gamma Specific) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized SARS-CoV-2 spike protein (Beta, Cat. No. SPN-C52Hk and Gamma, Cat. No. SPN-C52Hg) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS106) (Beta & Gamma Specific) (Cat. No. SPD-M416) with a linear range of 6-8 ng/mL. The antibody does not bind spike protein of WT (Cat. No. SPN-C52H7), Alpha (Cat. No. SPN-C52H6) and Delta (Cat. No. SPN-C52He) (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized SARS-CoV-2 Spike RBD protein B.1.1.529/Omicron (Cat. No. SPD-C522e), BA.2/Omicron ( Cat. No. SPD-C522g), BA.2.12.1/Omicron (Cat. No. SPD-C522q) and BA.3/Omicron (Cat. No. SPD-C522i) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS106) (Beta & Gamma Specific) (Cat. No. SPD-M416) with a linear range of 0.05-1.56 ng/mL. The antibody does not bind Spike RBD protein BA.4&BA.5/Omicron (Cat. No. SPD-C522r), Spike protein BA.4/Omicron (Cat. No. SPN-C5229) and BA.5/Omicron (Cat. No. SPN-C522e) (Routinely tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS106) (Beta & Gamma Specific) (Cat. No. SPD-M416) loaded on AMC Biosensor can bind SARS-CoV-2 S protein, His Tag (Beta, Cat. No. SPN-C52Hk) with an affinity constant of 19.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS106) (Beta & Gamma Specific) (Cat. No. SPD-M416) loaded on AMC Biosensor can bind SARS-CoV-2 S protein, His Tag (Gamma, Cat. No. SPN-C52Hg) with an affinity constant of 20.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS106) (Beta & Gamma Specific) (Cat. No. SPD-M416) loaded on AMC Biosensor does not bind SARS-CoV-2 S protein, His Tag (WT, Cat. No. SPN-C52H7) as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS106) (Beta & Gamma Specific) (Cat. No. SPD-M416) loaded on AMC Biosensor does not bind SARS-CoV-2 S protein, His Tag (Alpha, Cat. No. SPN-C52H6) as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

 

 

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