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 >  经MALS验证的蛋白>LAP (TGF-beta 1) >LAP-H82F8

Biotinylated Human LAP (TGF-beta 1) (C33S) Protein, Fc,Avitag™ (MALS verified)

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分子别名(Synonym)

LAP (TGF-beta 1),LAP (TGFB1),TGFB1,CED,DPD1,LAP,TGF-beta-1,TGFB

表达区间及表达系统(Source)

Biotinylated Human LAP (TGF-beta 1) (C33S), Fc,Avitag (LAP-H82F8) is expressed from human 293 cells (HEK293). It contains AA Leu 30 - Arg 278 (Accession # P01137-1(C33S)).

Predicted N-terminus: Leu 30

Request for sequence

蛋白结构(Molecular Characterization)

Online(Leu 30 - Arg 278) P01137-1 (C33S)

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 57.0 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human LAP (TGF-beta 1) (C33S), Fc,Avitag (Cat. No. LAP-H82F8) SDS-PAGE gel

Biotinylated Human LAP (TGF-beta 1) (C33S), Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Biotinylated Human LAP (TGF-beta 1) (C33S), Fc,Avitag (Cat. No. LAP-H82F8) MALS images

The purity of Biotinylated Human LAP (TGF-beta 1) (C33S), Fc,Avitag (Cat. No. LAP-H82F8) is more than 90% and the molecular weight of this protein is around 129-157kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT8-H52W4) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LAP (TGF-beta 1), Fc,Avitag (Cat. No. LAP-H82F8) with a linear range of 0.2-6 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-H52E1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LAP (TGF-beta 1), Fc,Avitag (Cat. No. LAP-H82F8) with a linear range of 0.2-13 ng/mL (Routinely tested).

Protocol

背景(Background)

Transforming growth factor beta 1 ( TGFB1) is also known as TGF-β1, CED, DPD1, TGFB. is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. The TGFB1 protein helps control the growth and division (proliferation) of cells, the process by which cells mature to carry out specific functions (differentiation), cell movement (motility), and the self-destruction of cells (apoptosis). The TGFB1 protein is found throughout the body and plays a role in development before birth, the formation of blood vessels, the regulation of muscle tissue and body fat development, wound healing, and immune system function. TGFB1 is particularly abundant in tissues that make up the skeleton, where it helps regulate bone growth, and in the intricate lattice that forms in the spaces between cells (the extracellular matrix). Within cells, this protein is turned off (inactive) until it receives a chemical signal to become active. TGFB1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGFB1. TGFB1 has been shown to interact with TGF beta receptor 1, LTBP1, YWHAE, EIF3I and Decorin.

 

 
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LAP (TGF-beta 1)靶点信息
英文全称:Transforming growth factor beta 1
中文全称:转化生长因子β1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:19详情
最高研发阶段:临床三期
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