登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>IL-18 R beta >ILB-H5256

Human IL-18 R beta Protein, Fc Tag

分子别名(Synonym)

IL-18 R beta,IL-1 R7,CD218b,CDw218b,IL-1 R7,IL-18 R beta,IL-18 receptor accessory protein,IL-18 receptor beta,IL18R beta,IL-18RAcP

表达区间及表达系统(Source)

Human IL-18 R beta Protein, Fc Tag (ILB-H5256) is expressed from human 293 cells (HEK293). It contains AA Phe 20 - Arg 356 (Accession # O95256-1).

Predicted N-terminus: Phe 20

Request for sequence

蛋白结构(Molecular Characterization)

IL-18 R beta Structure

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 64.9 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-18 R beta SDS-PAGE

Human IL-18 R beta Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-SPR

IL-18 R beta SPR

Biotinylated Human IL-18 R1, His,Avitag (Cat. No. IL1-H82E7) captured on Biotin CAP-Series S Sensor Chip can bind Human IL-18, Tag Free (Premixed with Human IL-18 R beta, Fc Tag, Cat. No. ILB-H5256) with an affinity constant of 5.87 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

背景(Background)

IL-18 R beta (IL-1R7), also known as IL18RAP (Interleukin 18 Receptor Accessory Protein), is a member of the IL-1 family. Within the IL18 receptor complex, does not mediate IL18-binding, but involved in IL18-dependent signal transduction, leading to NF-kappa-B and JNK activation

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
IL-18 R beta靶点信息
英文全称:Interleukin-18 receptor accessory protein
中文全称:IL18受体辅助蛋白
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:0详情
最高研发阶段:
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定