膜杰作
Star Staining
分子别名（Synonym）
THPO,MGC163194,MGDF,MKCSF,ML,MPLLG,TPO,THCYT1
表达区间及表达系统（Source）
Human Thrombopoietin Protein, Tag Free (THN-H5214) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Gly 353 (Accession # P40225-1).
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蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 35.6 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
无菌（Sterility）
The sterility testing was performed by membrane filtration method.
支原体（Mycoplasma）
Negative.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human Thrombopoietin Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-SPR
Human Thrombopoietin R, His Tag (Cat. No. THR-H52H7) captured on CM5 Chip via anti-His antibody can bind Human Thrombopoietin Protein, Tag Free (Cat. No. THN-H5214) with an affinity constant of 14.9 nM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
活性（Bioactivity）-Bioactivity CELL BASE
Human Thrombopoietin Protein, Tag Free (Cat. No. THN-H5214) stimulates proliferation of Mo7e cells. The specific activity of Human Thrombopoietin Protein, Tag Free is > 1.0ⅹ10^7 IU/mg (Routinely tested).
Protocol
背景（Background）
Thrombopoietin (TPO) is a 332 amino acid glycoprotein made primarily in the liver and act as the major physiological regulator of platelet production. TPO binds the TPO receptor, activates JAK and STAT pathways, thus stimulating megakaryocyte growth and platelet production. Additionally, further investigation uncovered that thrombopoietin is a critical cytokine promoting hematopoietic rebound after myeloablation and its transcripts are expressed by multiple cellular sources.
