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 >  经MALS验证的蛋白>OX40 Ligand >OXL-H5266

Human OX40 Ligand / TNFSF4 Protein, Fc Tag (active trimer) (MALS verified)

分子别名(Synonym)

OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1,CD134 ligand,CD134L

表达区间及表达系统(Source)

Human OX40 Ligand, Fc Tag (OXL-H5266) is expressed from human 293 cells (HEK293). It contains AA Gln 51 - Leu 183 (Accession # P23510-1).

Predicted N-terminus: Pro

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the N-terminus.

The protein has a calculated MW of 74.0 kDa. The protein migrates as 130-210 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human OX40 Ligand, Fc Tag (Cat. No. OXL-H5266) SDS-PAGE gel

Human OX40 Ligand, Fc Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Human OX40 Ligand, Fc Tag (Cat. No. OXL-H5266) MALS images

The purity of Human OX40 Ligand, Fc Tag (Cat. No. OXL-H5266) is more than 90% and the molecular weight of this protein is around 160-196 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human OX40 Protein, His Tag (MALS verified) (Cat. No. OX0-H5224) at 2 μg/mL (100 μL/well) can bind Human OX40 Ligand, Fc Tag (Cat. No. OXL-H5266) with a linear range of 0.1-1 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Cynomolgus / Rhesus macaque OX40, His Tag (Cat. No. OX0-C5220) at 2 μg/mL (100 μL/well) can bind Human OX40 Ligand, Fc Tag (Cat. No. OXL-H5266) with a linear range of 0.2-3 ng/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

Immobilized Biotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human OX40 Ligand, Fc Tag (Cat. No. OXL-H5266) with a linear range of 0.1-0.4 ng/mL (Routinely tested).

Protocol

背景(Background)

Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. TNFSF4 is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. Furthermore, similar to other TNF superfamily members, membrane-bound OX40 Ligand (TNFSF4) exists as a homotrimer. Human TNFSF4 shares 46% amino acid sequence identity with its mouse counterpart.

 

 
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OX40 Ligand靶点信息
英文全称:Tumor necrosis factor ligand superfamily member 4
中文全称:OX40配体
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:5详情
最高研发阶段:临床二期
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