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 >  Protein>OX40 Ligand >OXL-H5266

Human OX40 Ligand / TNFSF4 Protein, Fc Tag, premium grade

分子别名(Synonym)

OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1,CD134 ligand,CD134L

表达区间及表达系统(Source)

Human OX40 Ligand, Fc Tag, premium grade (OXL-H5266) is expressed from human 293 cells (HEK293). It contains AA Gln 51 - Leu 183 (Accession # P23510-1 ).

Predicted N-terminus: Pro

It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the N-terminus

The protein has a calculated MW of 74.0 kDa. The protein migrates as 140-200 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

无菌(Sterility)

The sterility testing was performed by membrane filtration method.

支原体(Mycoplasma)

Negative.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

OX40 Ligand SDS-PAGE

Human OX40 Ligand, Fc Tag, premium grade on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

OX40 Ligand SEC-MALS

The purity of Human OX40 Ligand, Fc Tag, premium grade (Cat. No. OXL-H5266) is more than 90% and the molecular weight of this protein is around 160-196 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

OX40 Ligand ELISA

Immobilized Human OX40 Protein, His Tag (Cat. No. OX0-H5224) at 2 μg/mL (100 μL/well) can bind Human OX40 Ligand, Fc Tag, premium grade (Cat. No. OXL-H5266) with a linear range of 0.1-1 ng/mL (QC tested).

Protocol

OX40 Ligand ELISA

Immobilized Cynomolgus / Rhesus macaque OX40, His Tag (Cat. No. OX0-C5220) at 2 μg/mL (100 μL/well) can bind Human OX40 Ligand, Fc Tag, premium grade (Cat. No. OXL-H5266) with a linear range of 0.2-3 ng/mL (Routinely tested).

Protocol

OX40 Ligand ELISA

Immobilized Biotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human OX40 Ligand, Fc Tag, premium grade (Cat. No. OXL-H5266) with a linear range of 0.1-0.4 ng/mL (Routinely tested).

Protocol

 

背景(Background)

Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. TNFSF4 is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. Furthermore, similar to other TNF superfamily members, membrane-bound OX40 Ligand (TNFSF4) exists as a homotrimer. Human TNFSF4 shares 46% amino acid sequence identity with its mouse counterpart.

 

前沿进展

 
 
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OX40 Ligand靶点信息
英文全称:Tumor necrosis factor ligand superfamily member 4
中文全称:OX40配体
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:6详情
最高研发阶段:临床三期
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