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Biotinylated Human FOLR2 Protein, His,Avitag™

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分子别名(Synonym)

FOLR2,BETA-HFR,FBP,PL-1,FR-BETA,FR-P3,FBP

表达区间及表达系统(Source)

Biotinylated Human FOLR2, His,Avitag (FO2-H82E8) is expressed from human 293 cells (HEK293). It contains AA Thr 17 - His 228 (Accession # P14207-1 ).

Predicted N-terminus: Thr 17

Request for sequence

蛋白结构(Molecular Characterization)

FOLR2 Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 28.2 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

FOLR2 SDS-PAGE

Biotinylated Human FOLR2, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

FOLR2 ELISA

Immobilized Folic acid-BSA conjugate at 5 μg/mL (100 μL/well) can bind Biotinylated Human FOLR2, His,Avitag (Cat. No. FO2-H82E8) with a linear range of 0.2-1 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-SPR

FOLR2 SPR

Immobilized Folic acid-BSA on CM5 Chip can bind Biotinylated Human FOLR2, His,Avitag (Cat. No. FO2-H82E8) with an affinity constant of 4.61 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

背景(Background)

Folate receptor beta is also known as Folate receptor 2, FBP, FOLR2, BETA-HFR, FBP/PL-1, FR-BETA, FR-P3, and is a member of the folate receptor (FOLR) family. and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. The FOLR2 protein was originally thought to exist only in placenta, but is also detected in spleen, bone marrow, and thymus. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells. It is upregulated on myeloid leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers. It is also upregulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma. FOLR2 is a marker for macrophages generated in the presence of M-CSF, but not GM-CSF. Its expression correlates with increased folate uptake ability. Folate conjugates of therapeutic drugs are a potential immunotherapy tool to target tumor-associated macrophages.

 

前沿进展

 
 
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FOLR2靶点信息
英文全称:Folate receptor beta
中文全称:叶酸受体β
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床三期
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