分子别名(Synonym)
uPAR,PLAUR,CD87,MO3
表达区间及表达系统(Source)
Human uPAR, Fc Tag (UPR-H5254) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Arg 303 (Accession # Q03405-1 ).
Predicted N-terminus: Leu 23
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries a human IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 57.8 kDa. The protein migrates as 65-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Human uPAR, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human uPAR, Fc Tag (Cat. No. UPR-H5254) is more than 90% and the molecular weight of this protein is around 124-151 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Anti-uPAR Antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Human uPAR, Fc Tag (Cat. No. UPR-H5254) with a linear range of 0.4-6 ng/mL (QC tested).
Protocol
背景(Background)
Urokinase plasminogen activator surface receptor (U-PAR) is also known as PLAUR, Monocyte activation antigen Mo3, CD antigen CD87. PLAUR contains three UPAR/Ly6 domains. U-PAR is expressed in neurons of the rolandic area of the brain (at protein level) and is also expressed in the brain. PLAUR / CD87 interacts with MRC2, SRPX2 and SORL1. PLAUR / UPAR acts as a receptor for urokinase plasminogen activator and plays a role in localizing and promoting plasmin formation. U-PAR mediates the proteolysis-independent signal transduction activation effects of U-PA.