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 >  Protein>DLL3 >DL3-H82E4

Biotinylated Human DLL3 Protein, His,Avitag™ (MALS verified)

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分子别名(Synonym)

Delta3,delta-like 3 (Drosophila),delta-like protein 3,DLL3,Pudgy,SCDO1,SCDO1delta3

表达区间及表达系统(Source)

Biotinylated Human DLL3, His,Avitag (DL3-H82E4) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Leu 492 (Accession # Q9NYJ7-1).

Predicted N-terminus: Ala 27

Request for sequence

蛋白结构(Molecular Characterization)

DLL3 Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 52.1 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

DLL3 SDS-PAGE

Biotinylated Human DLL3, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

DLL3 SEC-MALS

The purity of Biotinylated Human DLL3, His,Avitag (Cat. No. DL3-H82E4) is more than 90% and the molecular weight of this protein is around 51-69 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

DLL3 ELISA

Immobilized Biotinylated Human DLL3, His,Avitag (Cat. No. DL3-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-DLL3 Antibody (specific Binding EGFs of DLL3), Human IgG1 with a linear range of 0.1-4 ng/mL (QC tested).

Protocol

 

背景(Background)

Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands.May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. DLL3 protein is expressed on the surface of tumor cells but not in normal adult tissues.

 

前沿进展

 
 
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DLL3靶点信息
英文全称:Delta-like protein 3
中文全称:δ样蛋白-3
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:11详情
最高研发阶段:申请上市
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