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Biotinylated Mouse NKp46 / NCR1 / CD335 Protein, His,Avitag™

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利用生物素-链霉亲和素系统的高特异性和高灵敏度的结合特性,可将生物素化蛋白与链霉亲和素相关产品联合应用于各类实验,与非生物素体系相比,检测速度更快、结合能力更强、非特异背景更低。

分子别名(Synonym)

NCR1,LY94,CD335,NK-p46,hNKp46

表达区间及表达系统(Source)

Biotinylated Mouse NKp46, His,Avitag (NC1-M82H5) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Asn 255 (Accession # Q8C567-1 ).

Request for sequence

蛋白结构(Molecular Characterization)

NKp46 Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 30.3 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

NKp46 SDS-PAGE

Biotinylated Mouse NKp46, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

NKp46 ELISA

Immobilized Biotinylated Mouse NKp46, His,Avitag (Cat. No. NC1-M82H5) at 1 μg/mL (100 μL/well) on streptavidin (STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-NKp46 Antibody, Human IgG1 with a linear range of 1-31 ng/mL (QC tested).

Protocol

 

背景(Background)

Natural cytotoxicity triggering receptor 1 (NCR1) is also known as Natural killer cell p46-related protein (NK-p46), Lymphocyte antigen 94 homolog (LY94), CD antigen CD335, which belongs to the natural cytotoxicity receptor (NCR) family. NCR1 contains two Ig-like (immunoglobulin-like) domains. NCR1 interacts with CD247 and FCER1G. NCR1 / CD335 may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.

 

前沿进展

 
 
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NKp46靶点信息
英文全称:Natural cytotoxicity triggering receptor 1
中文全称:
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床二期
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