膜杰作
Star Staining
分子别名（Synonym）
Epstein-Barr virus (Herpesvirus 4),EBV Glycoprotein gp350,EBV GP350
表达区间及表达系统（Source）
Epstein-Barr virus (Herpesvirus 4) EBV Glycoprotein gp350 Protein, His Tag (GP0-E52H6) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Thr 470 (Accession # P03200-1).
Predicted N-terminus: Met 1
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 52.3 kDa. The protein migrates as 80-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally Trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Epstein-Barr virus (Herpesvirus 4) EBV Glycoprotein gp350 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Epstein-Barr virus (Herpesvirus 4) EBV Glycoprotein gp350 Protein, His Tag (Cat. No. GP0-E52H6) is more than 90% and the molecular weight of this protein is around 85-95 kDa verified by SEC-MALS.
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背景（Background）
Epstein-Barr virus (EBV), also designated human herpesvirus 4 (HHV-4), is a member of the herpesvirus family and is one of the most common human viruses. EBV binds to the cell surface receptor 2 (CR2) on human B cells using its major envelope glycoprotein 350 (gp350) and, as such, the EBV gp350 Envelope Protein, also designated the EBV envelope glycoprotein complex 250/350, is crucial in mediating the initial stages of EBV infection. The EBV gp350 Envelope Protein is expressed on virion envelope as well as EBV producer cells.
