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 >  Protein>3C (HRV) >3CC-N3133

HRV-3C Protease Cleavage Enzyme, GST Tag (active enzyme)

产品描述(Product Details)

HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue.

应用说明(Application)

  1. Removal of purification tags from fusion proteins

单位定义(Unit Definition)

One unit will cleave >95% of 100 µg control fusion protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4℃ for 16 h.

质量控制(Quility Control)

Endotoxin : Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH8.0 with trehalose as protectant.

Contact us for customized product form or formulation.

存储(Storage)

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

3C (HRV) SDS-PAGE

HRV-3C Protease Cleavage Enzyme, GST Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

 

活性(Bioactivity)

>1 Units/µg. One unit will cleave >95% of 100 µg control fusion protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4℃ for 16 h (QC tested).

文献引用(Citations)

 

前沿进展

 
 
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3C (HRV)靶点信息
英文全称:Picornain 3C protease
中文全称:Picornain 3C蛋白酶
种类:Human rhinovirus 1A
上市药物数量:0详情
临床药物数量:0详情
最高研发阶段:临床前
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