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 >  Protein>4-1BB Ligand >41L-R52D3

Rat 4-1BB Ligand / TNFSF9 Protein, His,Flag Tag (MALS verified)

分子别名(Synonym)

4-1BB Ligand,TNFSF9,CD137L

表达区间及表达系统(Source)

Rat 4-1BB Ligand His,Flag Tag (41L-R52D3) is expressed from human 293 cells (HEK293). It contains AA Pro 106 - Gly 308 (Accession # Q80WE6-1 ).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

4-1BB Ligand Structure

This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag

The protein has a calculated MW of 72.3 kDa. The protein migrates as 95-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

4-1BB Ligand SDS-PAGE

Rat 4-1BB Ligand His,Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

4-1BB Ligand SEC-MALS

The purity of Rat 4-1BB Ligand His,Flag Tag (Cat. No. 41L-R52D3) is more than 90% and the molecular weight of this protein is around 169-206 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

4-1BB Ligand ELISA

Immobilized Mouse 4-1BB, Fc Tag (Cat. No. 41B-M5258) at 1 μg/mL (100 μL/well) can bind Rat 4-1BB Ligand His,Flag Tag (Cat. No. 41L-R52D3) with a linear range of 5-39 ng/mL (QC tested).

Protocol

 

背景(Background)

Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9. 4-1BBL is the high affinity ligand of 4-1BB. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD). Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.

 

前沿进展

 
 
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4-1BB Ligand靶点信息
英文全称:Tumor necrosis factor ligand superfamily member 9
中文全称:肿瘤坏死因子配体超家族成员9
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床二期
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