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 >  Protein>FAP >FAP-M52H3

Mouse FAP Protein, His Tag, active dimer (MALS verified)

分子别名(Synonym)

FAP,FAPalpha,SIMP,Seprase,APCE

表达区间及表达系统(Source)

Mouse FAP, His Tag (FAP-M52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 761 (Accession # P97321 ).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

FAP Structure

This protein carries a polyhistidine tag at the N-terminus

The protein has a calculated MW of 87.1 kDa. The protein migrates as 80-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

FAP SDS-PAGE

Mouse FAP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

FAP SEC-MALS

The purity of Mouse FAP, His Tag (Cat. No. FAP-M52H3) is more than 90% and the molecular weight of this protein is around 180-190 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

FAP ELISA

Immobilized Mouse FAP, His Tag (Cat. No. FAP-M52H3) at 1 μg/mL (100 μL/well) can bind Anti-FAP Antibody, Mouse IgG2a (FAP5) with a linear range of 0.1-3 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-SPR

FAP SPR

Anti-FAP Antibody, Mouse IgG2a (FAP5) captured on CM5 chip via anti-mouse antibodies surface can bind Mouse FAP, His Tag (Cat. No. FAP-M52H3) with an affinity constant of 1.94 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

活性(Bioactivity)

Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >6500 pmol/min/µg (QC tested).

Protocol

 

背景(Background)

FAP (also known as seprase) is a Type II transmembrane serine protease. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences. Degrade also gelatin, heat-denatured type I collagen. Also has dipeptidyl peptidase activity, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.

 

前沿进展

 
 
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FAP靶点信息
英文全称:Fibroblast activation protein alpha
中文全称:成纤维细胞激活蛋白α
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:27详情
最高研发阶段:临床二期
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