膜杰作
Star Staining
分子别名（Synonym）
LGALS1,GAL1,GBP,Galectin-1,DKFZp686E23103
表达区间及表达系统（Source）
Human Galectin-1, Tag Free (GA1-H4113) is expressed from E. coli cells. It contains AA Met 1 - Asp 135 (Accession # P09382-1).
Predicted N-terminus: Met 1
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 14.71 kDa. The protein migrates as 13-15 kDa under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human Galectin-1, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human Galectin-1, Tag Free (Cat. No. GA1-H4113) is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-BLI
Loaded Biotinylated Human CD45, His,Avitag (Cat. No. CD5-H82E7) on SA Biosensor, can bind Human Galectin-1, Tag Free (Cat. No. GA1-H4113) with an affinity constant of 2.0 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
Galectin-1 (Gal-1), a member of the β-galactoside-binding animal lectin family, has a wide range of biological activities, which makes it an attractive target for medical applications. Gal-1 is ubiquitously but differentially expressed in normal and pathological animal tissues, and a variety of biological activities, including modulation of innate and adaptive immune responses, malignant progression of tumor cells, etc.
