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Rabbit CD40 / TNFRSF5 Protein, His Tag (MALS verified)

分子别名(Synonym)

CD40,Bp50,CDW40,MGC9013,TNFRSF5,p50

表达区间及表达系统(Source)

Rabbit CD40, His Tag (CD0-R52H9) is expressed from human 293 cells (HEK293). It contains AA Glu 77 - Arg 249 (Accession # G1SPC6-1).

Predicted N-terminus: Glu 77

蛋白结构(Molecular Characterization)

Online(Glu 77 - Arg 249) G1SPC6-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 20.9 kDa. The protein migrates as 28-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Rabbit CD40, His Tag (Cat. No. CD0-R52H9) SDS-PAGE gel

Rabbit CD40, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Rabbit CD40, His Tag (Cat. No. CD0-R52H9) MALS images

The purity of Rabbit CD40, His Tag (Cat. No. CD0-R52H9) was more than 90% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Rabbit CD40, His Tag (Cat. No. CD0-R52H9) at 5 μg/mL (100 μL/well) can bind Human CD40 Ligand, Fc Tag (active trimer) (MALS verified) (Cat. No. CDL-H5269) with a linear range of 0.2-1 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Rabbit CD40, His Tag (Cat. No. CD0-R52H9) at 5 μg/mL (100 μL/well) can bind Anti-CD40 Antibody, Human IgG2 with a linear range of 0.2-2 ng/mL (Routinely tested).

Protocol

背景(Background)

Tumor necrosis factor receptor superfamily member 5 (TNFRSF5) is also known as CD40, is a member of the TNF receptor superfamily. The expression of CD40 is diverse. TNFRSF5 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is the receptor for TNFSF5/CD40LG. Defects in CD40 are the cause of immunodeficiency with hyper-IgM type 3 (HIGM3).

 

前沿进展

 
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CD40靶点信息
英文全称:Tumor necrosis factor receptor superfamily member 5
中文全称:肿瘤坏死因子受体超家族成员5
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:17详情
最高研发阶段:临床二期
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