Rabbit CD40, His Tag (CD0-R52H9) is expressed from human 293 cells (HEK293). It contains AA Glu 77 - Arg 249 (Accession # G1SPC6-1).
Predicted N-terminus: Glu 77
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.9 kDa. The protein migrates as 28-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Rabbit CD40, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Rabbit CD40, His Tag (Cat. No. CD0-R52H9) was more than 90% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.
Immobilized Rabbit CD40, His Tag (Cat. No. CD0-R52H9) at 5 μg/mL (100 μL/well) can bind Human CD40 Ligand, Fc Tag (active trimer) (MALS verified) (Cat. No. CDL-H5269) with a linear range of 0.2-1 ng/mL (QC tested).
Immobilized Rabbit CD40, His Tag (Cat. No. CD0-R52H9) at 5 μg/mL (100 μL/well) can bind Anti-CD40 Antibody, Human IgG2 with a linear range of 0.2-2 ng/mL (Routinely tested).
Tumor necrosis factor receptor superfamily member 5 (TNFRSF5) is also known as CD40, is a member of the TNF receptor superfamily. The expression of CD40 is diverse. TNFRSF5 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is the receptor for TNFSF5/CD40LG. Defects in CD40 are the cause of immunodeficiency with hyper-IgM type 3 (HIGM3).