膜杰作
Star Staining
分子别名（Synonym）
SLC39A6,LIV-1,ZIP6,Zinc transporter ZIP6,ZIP-6
表达区间及表达系统（Source）
Human LIV-1, Fc Tag (LV1-H5255) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Trp 325 (Accession # Q13433-1).
Predicted N-terminus: Phe 29
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 60.1 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.05 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human LIV-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Human LIV-1, Fc Tag (Cat. No. LV1-H5255) at 1 μg/mL (100 μL/well) can bind Anti-LIV-1 Antibody, Human IgG1 with a linear range of 0.6-5 ng/mL (QC tested).
Protocol
背景（Background）
LIV-1 is also known as SLC39A6, ZIP-6 and Zinc transporter ZIP6. May act as a zinc-influx transporter. Highly expressed in the breast, prostate, placenta, kidney, pituitary and corpus callosum. Weakly expressed in heart and intestine. Also highly expressed in cells derived from an adenocarcinoma of the cervix and lung carcinoma. Up-regulated by estrogen in breast cancer cells lines.
