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 >  Protein>IL-11 >IL1-H82E3

Biotinylated Human IL-11 Protein, His,Avitag™ (MALS verified)

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分子别名(Synonym)

IL-11,Interleukin-11,AGIF,Oprelvekin,IL11

表达区间及表达系统(Source)

Biotinylated Human IL-11, His,Avitag (IL1-H82E3) is expressed from human 293 cells (HEK293). It contains AA Pro 22 - Leu 199 (Accession # P20809-1 ).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

IL-11 Structure

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 22.7 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-11 SDS-PAGE

Biotinylated Human IL-11, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

IL-11 SEC-MALS

The purity of Biotinylated Human IL-11, His,Avitag (Cat. No. IL1-H82E3) is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

IL-11 ELISA

Immobilized Biotinylated Human IL-11, His,Avitag (Cat. No. IL1-H82E3) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human IL-11 R alpha, Fc Tag (Cat. No. ILR-H5256) with a linear range of 0.4-25 ng/mL (QC tested).

Protocol

 

背景(Background)

Interleukin-11 (IL-11) is a pleiotropic cytokine that stimulates megakaryocytopoiesis, resulting in increased production of platelets, as well as activating osteoclasts, inhibiting epithelial cell proliferation and apoptosis, and inhibiting macrophage mediator production. These functions may be particularly important in mediating the hematopoietic, osseous and mucosal protective effects of IL-11. The cytokine also possesses anti-inflammatory activity, and has been proposed as a therapeutic agent in the treatment of chronic inflammatory diseases, such as Crohn's disease and rheumatoid arthritis. Although IL-11 was initially believed to be restricted to mammals, subsequent studies demonstrated it to be expressed in fish. Despite close similarity in gene structure and conservation of key amino acids between fish and mammalian IL-11, they share relatively low overall amino acid identity and may not necessarily be functionally analogous.

 

前沿进展

 
 
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IL-11靶点信息
英文全称:Interleukin-11
中文全称:白介素11
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:1详情
最高研发阶段:批准上市
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