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 >  Protein>IL-5 >IL5-M52H3

Mouse IL-5 Protein, His Tag (MALS verified)

分子别名(Synonym)

IL-5,TRF,IL5,Interleukin-5

表达区间及表达系统(Source)

Mouse IL-5, His Tag (IL5-M52H3) is expressed from human 293 cells (HEK293). It contains AA Met 21 - Gly 133 (Accession # P04401-1).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

Online(Met 21 - Gly 133) P04401-1

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 15.0 KDa. The protein migrates as 15 kDa,19 kDa and 23-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mouse IL-5, His Tag (Cat. No. IL5-M52H3) SDS-PAGE gel

Mouse IL-5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Mouse IL-5, His Tag (Cat. No. IL5-M52H3) MALS images

The purity of Mouse IL-5, His Tag (Cat. No. IL5-M52H3) is more than 90% and the molecular weight of this protein is around 37-47 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse IL-5, His Tag (Cat. No. IL5-M52H3) at 5 μg/mL (100 μL/well) can bind Human IL-5 R alpha, Fc Tag with a linear range of 5-39 ng/mL (QC tested).

Protocol

背景(Background)

Interleukin 5 (IL5) is an interleukin produced by type-2 T helper cells and mast cells. IL-5 is a 115-amino acid (in human, 133 in the mouse) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely interleukin 3 and GM-CSF), this glycoprotein in its active form is a homodimer. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma, wherein a large increase in the number of circulating, airway tissue, and induced sputum eosinophils have been observed. Given the high concordance of eosinophils and, in particular, allergic asthma pathology, it has been widely speculated that eosinophils have an important role in the pathology of this disease. Drugs that target IL-5 are mepolizumab and reslizumab.

 

 

前沿进展

 
 
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IL-5靶点信息
英文全称:Interleukin-5
中文全称:白细胞介素-5
种类:Homo sapiens
上市药物数量:2详情
临床药物数量:8详情
最高研发阶段:批准上市
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