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 >  其他蛋白>IL-1 beta >ILA-H525c

Human IL-1 beta / IL-1F2 Protein, Fc Tag (MALS verified)

分子别名(Synonym)

IL1B,IL-1BETA,IL1F2,IL-1β

表达区间及表达系统(Source)

Human IL-1 beta, Fc Tag (ILA-H525c) is expressed from human 293 cells (HEK293). It contains AA Ala 117 - Ser 269 (Accession # P01584-1).

Predicted N-terminus: Pro

Request for sequence

蛋白结构(Molecular Characterization)

Online(Ala 117 - Ser 269) P01584-1

This protein carries a human IgG1 Fc tag at the N-terminus.

The protein has a calculated MW of 43.8 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human IL-1 beta, Fc Tag (Cat. No. ILA-H525c) SDS-PAGE gel

Human IL-1 beta, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human IL-1 beta, Fc Tag (Cat. No. ILA-H525c) MALS images

The purity of Human IL-1 beta, Fc Tag (Cat. No. ILA-H525c) is more than 90% and the molecular weight of this protein is around 90-100 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL-1 RII, His Tag (Cat. No. IL2-H4226) at 5 μg/mL (100 μL/well) can bind Human IL-1 beta, Fc Tag (Cat. No. ILA-H525c) with a linear range of 4.9-39 ng/mL (QC tested).

Protocol

背景(Background)

Interleukin-1 beta (IL-1β) is also known as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene. IL-1β precursor is cleaved by caspase 1 (interleukin 1 beta convertase). Cytosolic thiol protease cleaves the product to form mature IL-1 beta. IL1β are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL1α and IL1β are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1β is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

 

 
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IL-1 beta靶点信息
英文全称:Interleukin-1 beta
中文全称:白细胞介素-1β
种类:Homo sapiens
上市药物数量:6详情
临床药物数量:14详情
最高研发阶段:批准上市
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