登录 | 注册    关注公众号  
微信公众号
搜索
 >  其他蛋白>IgG3 Fc >IG3-M5214

Mouse IgG3 Fc Protein, Tag Free (MALS verified)

分子别名(Synonym)

Ig gamma-3 chain C region,IgG,IgG3

表达区间及表达系统(Source)

Mouse IgG3 Fc Protein, Tag Free (IG3-M5214) is expressed from human 293 cells (HEK293). It contains AA Pro 98 - Pro 327 (Accession # P03987-2).

Request for sequence

蛋白结构(Molecular Characterization)

Online(Pro 98 - Pro 327) P03987-2

This protein carries no "tag".

The protein has a calculated MW of 26.1 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mouse IgG3 Fc Protein, Tag Free (Cat. No. IG3-M5214) SDS-PAGE gel

Mouse IgG3 Fc Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Mouse IgG3 Fc Protein, Tag Free (Cat. No. IG3-M5214) MALS images

The purity of Mouse IgG3 Fc Protein, Tag Free (Cat. No. IG3-M5214) is more than 90% and the molecular weight of this protein is around 60-70 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse IgG3 Fc Protein, Tag Free (Cat. No. IG3-M5214) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) with a linear range of 0.6-10 ng/mL (QC tested).

Protocol

背景(Background)

Immunoglobulin G3 (IgG3) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-3 chain C region (IgG3-Fc / IGHG3) contains two constant regions of IgG3 H chain (CH2, CH3).

 

前沿进展

 
 
货号/价格
文档
联系电话:
400-682-2521(全国)
010-53681107(北京)
021-31168505(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定