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 >  经MALS验证的蛋白>CD72 >CD2-H82E3

Biotinylated Human CD72 Protein, His,Avitag™ (MALS verified)

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分子别名(Synonym)

CD72,Lyb-2,Lyb2

表达区间及表达系统(Source)

Biotinylated Human CD72, His,Avitag (CD2-H82E3) is expressed from human 293 cells (HEK293). It contains AA Arg 117 - Asp 359 (Accession # P21854-1).

Predicted N-terminus: Arg 117

Request for sequence

蛋白结构(Molecular Characterization)

Online(Arg 117 - Asp 359) P21854-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 31.7 kDa. The protein migrates as 34-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human CD72, His,Avitag (Cat. No. CD2-H82E3) SDS-PAGE gel

Biotinylated Human CD72, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Biotinylated Human CD72, His,Avitag (Cat. No. CD2-H82E3) MALS images

The purity of Human Siglec-3, His Tag (Cat. No. CD3-H5226) is more than 95% and the molecular weight of this protein is around 75-100 kDa verified by SEC-MALS.

Report

背景(Background)

B-cell differentiation antigen CD72 is also known as Lyb-2. CD72 contains one C-type lectin domain. CD72 is a protein active in the immune system of animals. CD72 consists of two identical halves, each of about 39-43 kD, and is a C-type lectin. Its primarily locus of expression is B-cells, where it appears to mediate aspects of B-cell - T-cell interaction. CD72 plays a role in B-cell proliferation and differentiation. CD72 is a ligand for CD5.

 

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