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 >  Protein>Fc gamma RIII / CD16 >FC6-M82E0

Biotinylated Mouse Fc gamma RIII / CD16 Protein, His,Avitag™ (MALS & SPR verified)

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分子别名(Synonym)

FCGR3

表达区间及表达系统(Source)

Biotinylated Mouse CD16, His,Avitag (FC6-M82E0) is expressed from human 293 cells (HEK293). It contains AA Leu 32 - Thr 215 (Accession # Q5D5I8-1).

Predicted N-terminus: Leu 32

Request for sequence

蛋白结构(Molecular Characterization)

Online(Leu 32 - Thr 215) Q5D5I8-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 24.6 kDa. The protein migrates as 35-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Mouse CD16, His,Avitag (Cat. No. FC6-M82E0) SDS-PAGE gel

Biotinylated Mouse CD16, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Biotinylated Mouse CD16, His,Avitag (Cat. No. FC6-M82E0) MALS images

The purity of Biotinylated Mouse CD16, His,Avitag (Cat. No. FC6-M82E0) is more than 90% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.

Report

活性(Bioactivity)-SPR

Biotinylated Human SPR

Biotinylated Mouse CD16, His,Avitag (Cat. No. FC6-M82E0) captured on Biotin CAP- Series S Sensor Chip can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with an affinity constant of 0.345 μM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

 

背景(Background)

CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody­dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.

 

文献引用(Citations)

 

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Fc gamma RIII / CD16靶点信息
英文全称:Low affinity immunoglobulin gamma Fc region receptor III-A
中文全称:低亲和力免疫球蛋白γFc段受体III-A
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:12详情
最高研发阶段:临床二期
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