膜杰作
Star Staining
分子别名（Synonym）
C7orf15,C7orf15MGC138295,CD112R,MGC104322,MGC138297,MGC2463,PVRIG,CD112 receptor
表达区间及表达系统（Source）
Cynomolgus PVRIG, Mouse IgG2a Fc Tag (PVG-C5253) is expressed from human 293 cells (HEK293). It contains AA Thr 41 - Asp 171 (Accession # A0A2K5WVV8-1).
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蛋白结构（Molecular Characterization）
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 40.7 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Cynomolgus PVRIG, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Cynomolgus PVRIG, Mouse IgG2a Fc Tag (Cat. No. PVG-C5253) is more than 90% and the molecular weight of this protein is around 85-105 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human Nectin-2, His Tag (Cat. No. PV2-H52E2) at 5 μg/mL (100 μL/well) can bind Cynomolgus PVRIG, Mouse IgG2a Fc Tag (Cat. No. PVG-C5253) with a linear range of 2-20 ng/mL (QC tested).
Protocol
背景（Background）
Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family. The CD112R gene encodes a putative single transmembrane protein, which is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. Notably, the intracellular domain of phatases. The extracellular domain sequence of human and mouse CD112R have 65.3% similarity. CD112R may act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals.
