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 >  Kits>Spike RBD >RAS-T017

Anti-SARS-CoV-2 Antibody IgG4 Titer Serologic Assay Kit

For research use only.

IDComponentsSize
RAS017-C01Pre-coated with SARS-CoV-2 Spike RBD Microplate1 plate
RAS017-C02Anti-SARS-CoV-2 Antibody (Control, IgG4)10 μg
RAS017-C03HRP-Mouse anti-Human IgG450 μL
RAS017-C0410 x Washing Buffer 50 mL
RAS017-C05Dilution Buffer50 mL
RAS017-C06Substrate Solution12 mL
RAS017-C07Stop Solution7 mL

背景(Background)

The ongoing SARS-COV-2 pandemics has sickened 3.7 million people around the world, claiming over 1,000,000 lives. To deal with this global public health crisis, unprecedented efforts are being made to study the virus, SARS-CoV-2. At present, there are 193 new crown vaccine projects registered with the World Health Organization are being developed. To support these studies, ACRO has developed Anti-SARS-CoV-2 subtype antibody Serologic Assay kit including:IgG1, IgG2 IgG3, IgG4.

应用说明(Application)

This kit is developed for serologic test for IgG4 titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.

It is for research use only.

重构方法(Reconstitution)

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储 & 运输(Storage & Shipping)

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Shipping Statement

原理(Assay Principles)

This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in serum by SARS-CoV-2 Spike RBD. The Kit consists of Pre-coated with SARS-CoV-2 Spike RBD Microplate, an Anti-SARS-CoV-2 Antibody (Control, IgG4), an HRP-Anti-Human IgG4 secondary antibody.

Your experiment will include 4 simple steps:

a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.

b) Add diluted Secondary antibody HRP-Mouse-Anti-Human IgG4 to the plate. The Secondary antibody is diluted by Dilution Buffer.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

 

典型数据-Typical Data Please refer to DS document for The assay protocol.

Spike RBD TYPICAL DATA

Detection of Anti-SARS-CoV-2 Antibody, Human IgG4 titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG4 in 1:50 human serum. Detection was performed using HRP-Conjugated Anti-human IgG4 antibody with sensitivity of 4.9 ng/mL (QC tested).

 

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