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 >  Protein>CEACAM-5 >CE5-H5253

Human CEACAM-5 Protein, Fc Tag (HPLC verified)

分子别名(Synonym)

CEACAM-5,CD66e,CEA,Meconium antigen 100

表达区间及表达系统(Source)

Human CEACAM-5, Fc Tag (CE5-H5253) is expressed from human 293 cells (HEK293). It contains AA Lys 35 - Ala 685 (Accession # P06731-1 ).

Predicted N-terminus: Lys 35

Request for sequence

蛋白结构(Molecular Characterization)

CEACAM-5 Structure

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 97.8 kDa. The protein migrates as 150-180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

CEACAM-5 SDS-PAGE

Human CEACAM-5, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-HPLC

CEACAM-5 SEC-HPLC

The purity of Human CEACAM-5, Fc Tag (Cat. No. CE5-H5253) was greater than 90% as determined by SEC-HPLC.

 

活性(Bioactivity)-ELISA

CEACAM-5 ELISA

Immobilized Human CEACAM-5, Fc Tag (Cat. No. CE5-H5253) at 1 μg/mL (100 μL/well) can bind Labetuzumab with a linear range of 1-10 ng/mL (QC tested).

Protocol

 

背景(Background)

Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is also Carcinoembryonic antigen (CEA), Meconium antigen 100, CD antigen CD66e, CEACAM5 belongs to the immunoglobulin superfamily and CEA family. CEACAM5 contains seven Ig-like (immunoglobulin-like) domains. CEACAM5 is homodimer protein which binding of E.coli Dr adhesins leads to dissociation of the homodimer. CEACAM5 is cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEACAM5 is receptor for E.coli Dr adhesins.

 

前沿进展

 
 
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CEACAM-5靶点信息
英文全称:Carcinoembryonic antigen related cell adhesion molecule 5
中文全称:癌胚抗原相关细胞粘附分子5
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:10详情
最高研发阶段:临床三期
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