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Biotinylated Human Claudin-18.2 Protein, His,Avitag™ (active membrane protein)

分子别名(Synonym)

Claudin-18.2,CLDN18,Claudin-18

表达区间及表达系统(Source)

Biotinylated Human Claudin-18.2, His,Avitag (CL2-H82E3) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Ala 200 (Accession # P56856-2).

Predicted N-terminus: Met

蛋白结构(Molecular Characterization)

Online(Met 1 - Ala  200) P56856-2

This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 24.8 kDa.
It is produced as active membrane protein with full ECD epitopes, and contains both the ECL1 and ECL2.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>60% as determined by SDS-PAGE.

制剂(Formulation)

Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep Claudin-18.2 soluble and active, under no circumastance should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 3 months under sterile conditions.
 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Biotinylated Human Claudin-18.2, His,Avitag (Cat. No. CL2-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (in presence of DDM and CHS) (QC tested).

Protocol

背景(Background)

Claudins (CLDNs) are a family of proteins that form tight junctions and maintain the polarity of epithelial and endothelial cells. CLDN18 is specifically expressed in the stomach and lung. Of the two CLDN18 isoform transcripts produced by alternative splicing, CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific. CLDN18.2 is a highly selective gastric lineage antigen expressed exclusively on short-lived differentiated gastric epithelial cells where it has only limited accessibility to antibody drugs.14,15 CLDN18.2 is maintained during the course of malignant transformation and thus frequently displayed on the surface of human gastric cancer cells.

 

前沿进展

 
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Claudin-18.2靶点信息
英文全称:Claudin-18 splice variant 2
中文全称:密蛋白-18剪接变体2
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:18详情
最高研发阶段:临床三期
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