Rabbit BCMA, His Tag (BCA-R52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 1 - Asn 52 (Accession # G1SFN4-1).
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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 7.9 kDa. The protein migrates as 12 kDa and 14 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Rabbit BCMA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human APRIL, Fc Tag (Cat. No. APL-H5267) at 5 μg/mL (100 μL/well) can bind Rabbit BCMA, His Tag (Cat. No. BCA-R52H4) with a linear range of 2-5 ng/mL (QC tested).
Immobilized Rabbit BCMA, His Tag (Cat. No. BCA-R52H4) at 2 μg/mL (100 μL/well) can bind Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) with a linear range of 0.4-6 ng/mL (Routinely tested).
Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.