膜杰作
Star Staining
分子别名（Synonym）
Glucagon R, GCGR,Glucagon receptor
表达区间及表达系统（Source）
Human GCGR, His Tag (GCR-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Lys 136 (Accession # P47871-1).
Predicted N-terminus: Ala 26
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 14.9 kDa. The protein migrates as 28-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human GCGR, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human GCGR, His Tag (Cat. No. GCR-H52H3) is more than 85% and the molecular weight of this protein is around 23-33 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human GCGR, His Tag (Cat. No. GCR-H52H3) at 1 μg/mL (100 μL/well) can bind Human Anti-GCGR (Volagidemab), Human IgG2 with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
背景（Background）
G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.
