登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>CD3E & CD3G >CDG-H52W5

Human CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Tag Free (MALS verified)

分子别名(Synonym)

CD3 epsilon & CD3 gamma,CD3E & CD3G

表达区间及表达系统(Source)

Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (CDG-H52W5) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P07766-1 (CD3E) & P09693-1 (CD3G)).

Predicted N-terminus: Asp 23 (CD3E) & Gln 23 (CD3G)

Request for sequence

蛋白结构(Molecular Characterization)

CD3E & CD3G Structure

Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3G, has a calculated MW of 16.9 kDa (CD3E) and 14.6 kDa (CD3G). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3G contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 19 kDa and 21-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

CD3E & CD3G SDS-PAGE

Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

CD3E & CD3G SEC-MALS

The purity of Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) is more than 90% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

CD3E & CD3G ELISA

Immobilized Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with a linear range of 0.1-3 ng/mL (QC tested).

Protocol

CD3E & CD3G ELISA

Immobilized Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) at 5 μg/mL (100 μL/well) can bind Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) with a linear range of 0.039-0.156 μg/mL (Routinely tested).

Protocol

CD3E & CD3G ELISA

Immobilized UCHT1 at 1 μg/mL (100 μL/well) can bind Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) with a linear range of 0.8-3 ng/mL (Routinely tested).

Protocol

CD3E & CD3G ELISA

Immobilized Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) at 1 μg/mL (100 μL/well) can bind UCHT1 with a linear range of 0.4-3 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-SPR

CD3E & CD3G SPR

Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) (Cat. No. CDE-M531) captured on CM5 chip via anti-mouse antibodies surface can bind Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) with an affinity constant of 13.4 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

CD3E & CD3G SPR

Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) captured on CM5 chip via anti-mouse antibodies surface can bind Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) with an affinity constant of 810 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

背景(Background)

T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

 

前沿进展

 
 
货号/价格
产品推荐
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定