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SARS S protein (R667A, K968P, V969P), His Tag (MALS verified)

分子别名(Synonym)

Spike,S protein,Spike glycoprotein,S glycoprotein

表达区间及表达系统(Source)

SARS S protein (R667A, K968P, V969P), His Tag (SPN-S52H6) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1 (R667A, K968P, V969P)). The recombinant protein is expressed with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (K968P, V969P) and alanine substitutions (R667A) are introduced to stabilize the trimeric prefusion state of SARS-CoV S protein and abolish the furin cleavage site, respectively.

Predicted N-terminus: Ser 14

Request for sequence

蛋白结构(Molecular Characterization)

Spike protein Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 136.3 kDa. The protein migrates as 190-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Spike protein SDS-PAGE

SARS S protein (R667A, K968P, V969P), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Spike protein SEC-MALS

The purity of SARS S protein (R667A, K968P, V969P), His Tag (Cat. No. SPN-S52H6) is more than 90% and the molecular weight of this protein is around 508-562 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Spike protein ELISA

Immobilized SARS S protein (R667A, K968P, V969P), His Tag (Cat. No. SPN-S52H6) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-6 ng/mL (QC tested).

Protocol

Spike protein ELISA

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind SARS S protein (R667A, K968P, V969P), His Tag (Cat. No. SPN-S52H6) with a linear range of 2-20 ng/mL (Routinely tested).

Protocol

Spike protein ELISA

Immobilized Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind SARS S protein (R667A, K968P, V969P), His Tag (Cat. No. SPN-S52H6) with a linear range of 5-78 ng/mL (Routinely tested).

Protocol

Spike protein ELISA

Immobilized SARS S protein (R667A, K968P, V969P), His Tag (Cat. No. SPN-S52H6) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (Routinely tested).

Protocol

 

背景(Background)

The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

文献引用(Citations)

 

前沿进展

 
 

Purchaser Notification

Important Licensing Information: This product may be covered by Limited Use Label License(s). By use of this product, you accept the terms and conditions of all applicable Limited Use Label License(s).

Limited Use Label License No. 17: Prefusion Coronavirus Spike Proteins

This product and its use is the subject of one or more U.S. Provisional Patent Application Nos. 62/412,703; 62/972,886; U.S. Patent Application No. 16/344,774 and corresponding patents and applications in other countries. This product is sold under licenses from The National Institute of Allergy & Infectious Diseases/National Institutes of Health. Rights to use this product are limited to research only (including Non-clinical Diagnostic Kits), and expressly exclude the right to use in human therapeutics, vaccines and clinical diagnostics and any use involving the administration of this product to humans.

 
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Spike protein靶点信息
英文全称:Coronavirus spike glycoprotein
中文全称:冠状病毒刺突糖蛋白
种类:Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
上市药物数量:9详情
临床药物数量:68详情
最高研发阶段:批准上市
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