登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>Spike RBD >SPD-C52Hp

SARS-CoV-2 (COVID-19) S protein RBD (K417N, E484K, N501Y), His Tag (MALS verified)

分子别名(Synonym)

Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD

表达区间及表达系统(Source)

SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), His Tag (SPD-C52Hp) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (K417N, E484K, N501Y)). The K417N / E484K / N501Y mutations were identified in the SARS-CoV-2 Beta variant (Pango lineage: B.1.351; other names: 20H/501Y.V2).

Predicted N-terminus: Arg 319

Request for sequence

蛋白结构(Molecular Characterization)

Spike RBD Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 26.6 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Spike RBD SDS-PAGE

SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Spike RBD SEC-MALS

The purity of SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), His Tag (Cat. No. SPD-C52Hp) is more than 90% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Spike RBD ELISA

Immobilized SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), His Tag (Cat. No. SPD-C52Hp) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-3 ng/mL (QC tested).

Protocol

Spike RBD ELISA

Immobilized SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), His Tag (Cat. No. SPD-C52Hp) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM128) (Cat. No. SPD-M128) with a linear range of 0.1-0.8 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-SPR

Spike RBD SPR

Human ACE2, Fc Tag (Cat. No. AC2-H5257) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), His Tag (Cat. No. SPD-C52Hp) with an affinity constant of 3.27 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

背景(Background)

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

文献引用(Citations)

 

前沿进展

 
 
货号/价格
产品推荐
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定