| ID | Components | Size |
| RAS020-C01 | Pre-coated with Anti-SARS-CoV-2 Spike Protein Antibody Microplate | 1 plate |
| RAS020-C02 | SARS-CoV-2 Spike Protein | 10 μg |
| RAS020-C03 | Biotin-Anti-SARS-CoV-2 Spike Protein Antibody | 10 μg |
| RAS020-C04 | Streptavidin-HRP | 10 μg |
| RAS020-C05 | 10xWashing Buffer | 50 mL |
| RAS020-C06 | Dilution Buffer | 50 mL |
| RAS020-C07 | Substrate Solution | 12 mL |
| RAS020-C08 | Stop Solution | 7 mL |
Background/背景
The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of SARS-CoV-2 Spike protein is urgently needed to accelerate the development of COVID-19 vaccines.
Application/应用
This kit is developed for detecting SARS-CoV-2 Spike Protein in the sample.
It is for research use only.
Reconstitution/重构方法
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage/存储
The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.
It is recommended not to freeze thaw more than 3 times for powder.
Assay Principles/原理
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike Protein. The kit consists of microplate pre-coated with Anti-SARS-CoV-2 Spike protein Antibody, an SARS-CoV-2 Spike Protein as Control, an biotin-Anti-SARS-CoV-2 Spike protein Antibody, HRP-Streptavidin and buffers.
Your experiment will include 6 simple steps:
a) All reagents were returned to room temperature(20℃-25℃) before use.
b) Add your sample to the plate, take the SARS-CoV-2 Spike protein as Control sample. The samples and Control sample are diluted by Dilution Buffer.
c) Add a diluted Secondary antibody biotin-Anti-SARS-CoV-2 Spike protein Antibody to the plate. The Secondary antibody is diluted by Dilution Buffer.
d) Add a diluted Streptavidin-HRP to the plate.
e) Wash the plate and add TMB or other colorimetric HRP substrate.
f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
Typical Data/典型数据
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike protein. The kit consists of pre-coated Anti-SARS-CoV-2 Spike Protein Antibody Microplate, SARS-CoV-2 Spike protein as Control, biotin-Anti-SARS-CoV-2 Spike protein antibody and HRP-Streptavidin. Detection is performed using HRP-Streptavidin with sensitivity of 200 pg/mL.
Protocol
