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SARS-CoV-2 Spike RBD Titer Assay Kit

For research use only.

IDComponentsSize
RAS021-C01Pre-coated with Anti-SARS-CoV-2 Spike RBD Antibody Microplate1 plate
RAS021-C02SARS-CoV-2 Spike RBD10 μg
RAS021-C03Biotin-Anti-SARS-CoV-2 Spike RBD Antibody10 μg
RAS021-C04Streptavidin-HRP10 μg
RAS021-C0510 x Washing Buffer 50 mL
RAS021-C06Dilution Buffer50 mL
RAS021-C07Substrate Solution12 mL
RAS021-C08Stop Solution7 mL

Background/背景

The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of SARS-CoV-2 Spike Protein RBD is urgently needed to accelerate the development of COVID-19 vaccines.

Application/应用

This kit is developed for detecting SARS-CoV-2 Spike RBD in the sample.

It is for research use only.

Reconstitution/重构方法

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage/存储

The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.

It is recommended not to freeze thaw more than 3 times for powder.

This product is stable under storage conditions: ● 12 months in sealed state. ● Used within 1 months after opening.

Assay Principles/原理

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike RBD. The kit consists of microplate pre-coated with Anti-SARS-CoV-2 Spike RBD Antibody, an SARS-CoV-2 Spike RBD as Control, an biotin-Anti-SARS-CoV-2 Spike RBD Antibody, HRP-Streptavidin and buffers.

Your experiment will include 6 simple steps:

a)All reagents were returned to room temperature(20℃-25℃) before use.

b) Add your sample to the plate, take the SARS-CoV-2 Spike RBD as Control sample. The samples and Control sample are diluted by Dilution Buffer.

c) Add a diluted Secondary antibody biotin-Anti-SARS-CoV-2 Spike RBD Antibody to the plate. The Secondary antibody is diluted by Dilution Buffer.

d) Add a diluted Streptavidin-HRP to the plate.

e) Wash the plate and add TMB or other colorimetric HRP substrate.

f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

 

Typical Data/典型数据

Biotinylated Human ELISA

This assay kit employs a standard sandwich-ELISA format providing a rapid detection of SARS-CoV-2 Spike RBD. The kit consists of pre-coated Anti-SARS-CoV-2 Spike RBD Antibody Microplate, SARS-CoV-2 Spike RBD as Control, biotin-Anti-SARS-CoV-2 Spike RBD antibody and HRP-Streptavidin. Detection is performed using HRP-Streptavidin with sensitivity of 12 pg/mL (QC tested).

Protocol

Biotinylated Human SPR

SARS-CoV-2 Spike RBD Titer Assay Kit (Cat.No.RAS-A021) can potently to detect the all SARS-CoV-2 Variants of Concern (VOCs), including Alpha (Cat.No.SPD-C5225), Beta (Cat.No.SPD-C52Hp), Gamma (Cat.No.SPD-C52Hr) and Delta (Cat.No.SPD-C52Hh).

Protocol

前沿进展

 
 
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