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Biotinylated Mouse CD3 epsilon&CD3 gamma Heterodimer Protein, His,Avitag™&Flag Tag (MALS verified)

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利用生物素-链霉亲和素系统的高特异性和高灵敏度的结合特性,可将生物素化蛋白与链霉亲和素相关产品联合应用于各类实验,与非生物素体系相比,检测速度更快、结合能力更强、非特异背景更低。

分子别名(Synonym)

CD3 epsilon & CD3 gamma,CD3E & CD3G

表达区间及表达系统(Source)

Biotinylated Mouse CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (CDG-M58D2) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 108 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P22646-1(CD3E) & P11942-1(CD3G)).

Predicted N-terminus: Asp 23 (CD3E) & Gln 23 (CD3G)

Request for sequence

蛋白结构(Molecular Characterization)

Online(Asp 23 - Asp 108 (CD3E) & Gln 23 - Ser 116 (CD3G)) P22646-1 (CD3E) & P11942-1 (CD3G)

Biotinylated Mouse CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 16.8 kDa (CD3E) and 15.2 kDa (CD3G). Subunit CD3E carries a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G is fused with a flag tag at the C-terminus. As a result of glycosylation, the protein migrates as 20-23 kDa under reducing (R) condition, and 35-40 kDa under non-reducing (NR) condition (SDS-PAGE).

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Mouse CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. CDG-M58D2) SDS-PAGE gel

Biotinylated Mouse CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Biotinylated Mouse CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. CDG-M58D2) MALS images

The purity of Biotinylated Mouse CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. CDG-M58D2) is more than 90% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized CD3E Monoclonal Antibody (145-2C11) at 1 μg/mL (100 μL/well) can bind Biotinylated Mouse CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. CDG-M58D2) with a linear range of 5-78 ng/mL (QC tested).

Protocol

背景(Background)

T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

 

 

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