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 >  Antibody>FMC63 >FM3-BY54

Biotinylated Monoclonal Anti-FMC63 Antibody, Mouse IgG1, Avitag™ (Y45) DMF

抗体来源(Source)

Biotinylated Monoclonal Anti-FMC63 Antibody, Mouse IgG1, Avitag (Cat. No. FM3-BY54) is expressed from human 293 cells.

亚型(Isotype)

Mouse IgG1/kappa

特异性(Specificity)

Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

应用(Application)

Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression).

*The Isotype control (Cat. No. DNP-BM190) is sold separatelyand you can follow this link for product information.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 6 months under sterile conditions after reconstitution.
 

活性(Bioactivity)-FACS

FMC63 FACS

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) Biotinylated Monoclonal Anti-FMC63 Antibody, Mouse IgG1, Avitag (Cat. No. FM3-BY54) and isotype control respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).

Protocol

 

背景(Background)

FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.

文献引用(Citations)

 

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